By the end of this course you should be able to:

  • accurately, safely and appropriately use all the equipment regularly used in DNA manipulation, including balances, pipettes, electrophoresis and centrifuges
  • prepare chemical solution and reagents to the precision appropriate to the task
  • demonstrate knowledge of the biochemical basis underpinning the molecular biology techniques
  • independently clone any gene into a plasmid vector (from RNA extraction, reverse transcription, polymerase chain reaction, ligation, bacterial transformation, to DNA extraction, DNA mapping and primer design)
  • Transfect plasmids and silencing RNAs to over-express or knock down protein expression in a primary cell line, extract protein, assess and quantify expression using Western blotting
  • carry out molecular biology experiments and interpret the results, designing a strategy to circumvent potential failed experiments